The Bustamante Lab | UC Berkeley | UniPR
Assemblies: puller Head

 

puller Head

Description

Holds glass pipette tube inside filament loop. When filament gets hot, glass melts , puller fixer (clamp dog) drops , sliding down puller posts, thus drawing fine point on pipette tube.

A 2-loop filament made from 35mm of 0.25mm dia. Pt90-Ir10 wire formed on a 1.8 mm diameter mandrel will glow orange-hot when the current reaches 3.5 Amperes and the voltage drop is 1.45 Volts.  This power gives sufficient heat to melt glass and form a pipette tip. 
Type Assembly
Pictures
Picture
puller filament holder

puller filament holder
Picture
puller head complete

puller head complete
Picture
Pipette puller head

Pipette puller head
Picture
Pipette puller head

Pipette puller head
Picture
puller fixer clamp
6-32 socket-head screw, plastic thumb-screw knob, fixer clamp. Round brass nut ring is threaded onto screw and glued in place with epoxy so that unscrewing knob will pull fixer clamp away from Slider or Top piece.

puller fixer clamp
Parents
Parts
NameManufacturerVendorRequiredStockCost
Knob for #6 capScrewMcMaster Carr5$0.12
Platinum wire0.3 mm Alfa Aesar4 cm$3.60
puller Bottomcustom shopVarious machine shops1$50.00
Puller Filament Mountcustom shopVarious machine shops1$0.00
puller Fixercustom shopVarious machine shops1$33.00
puller Holdercustom shopVarious machine shops1$83.00
puller Postcustom shopVarious machine shops2$33.00
puller Slidercustom shopVarious machine shops1$66.00
puller Topcustom shopVarious machine shops1$66.00
Screw, SS Allen-cap6-32x3/4McMaster Carr5$0.06
Screw, SS Pan-Head6-32x1/4McMaster Carr4$0.05
Spacer, BrassThreaded 6-32 x 1/2"McMaster Carr2$0.56
Washer, Fender5/8" o.d. for #8 screwsMcMaster Carr2$0.08
Estimated cost: $380.81
 

List all the parts for "puller Head"

last changed 2023/08/05 00:43

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