The Bustamante Lab
: Buffer bottle, pressure
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Buffer bottle, pressure
glass blowing shop
Cost per unit
Uses same liquid scintilation vial as "Buffer Bottle, Waste" except modified by glass blower.
Must be borosilicate glass.
Must use cone-sealing lids (not foil lids).
(A) buffer bottle glass blown using borosilicate glass vial and 4-mm tubes.JPG
(B) scratch and shorten lower tube using a triangular file and cut off the end of a 1ml syringe at the 0.1ml marking with razor blade.JPG
(C) heat lower extension using a bunsen burner.JPG
(D) you will see orange flames when it has heated enough.JPG
(E) attach the syringe tip to the extension by letting it melt onto hot glass.JPG
(F) the finished product with its cone-type pressure sealing cap.jpg
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